Any two of the following would do justice: - DNA Helicase unwinds the double helix by disrupting H bonds that bind the nitrogenous bases - Single-stranded binding proteins prevent the separated DNA strands from re-attaching - RNA Primer made up of multiple nitrogenous bases which are attached to the template strand and initiate the building of a new complementary strand i.e replication - Primase builds RNA primers - DNA Polymerase I removes RNA primers and replaces them with appropriate DNA nucleotides - DNA Polymerase III creates complementary strands of DNA, attach to an RNA primer, and add nucleotides to the 3' end - DNA Ligase joins Okazaki fragments together by phosphodiester bonds - DNA Gyrase relieves tension produced when DNA double helix is unwound by helicase
